When you first use the Microfluidizer, you will see some liquid in the sample chamber, as shown in Figure 2. This is the storage solution – 70% isopropanol. Before you add your sample to the sample chamber, you will want to wash out the sample chamber extensively with water. To do so, flip the valve as shown in Figure 3. This will start the machine. Note that when you are washing the sample chamber, never let air get into the machine. You should never let the machine run if the chamber is empty/dry. Always make sure that liquid is flowing through the machine to prevent air bubbles from getting into the system.