Mar 18, 2024

Public workspacePROTOCOL for “Systemic inflammation triggers long-lasting neuroinflammation and accelerates neurodegeneration in a rat model of Parkinson’s disease overexpressing human α-synuclein” V.2

DOI
dx.doi.org/10.17504/protocols.io.j8nlkoj65v5r/v2
  • 1Department of Experimental Neuroscience, Santa Lucia Foundation IRCCS, Rome, Italy;
  • 2Department of Systems Medicine, University of Rome Tor Vergata, Rome, Italy;
  • 3Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD, United States;
  • 4Laboratory of Resolution of Neuroinflammation, Santa Lucia Foundation IRCCS, Rome, Italy;
  • 5Institute of Translational Pharmacology, National Research Council, Rome, Italy
Nicola Biagio Mercuri
Open access
DOI: dx.doi.org/10.17504/protocols.io.j8nlkoj65v5r/v2
Collection Citation: mariangela.massarocenere, Valerio Chiurchiù, Nicola Biagio Mercuri 2024. PROTOCOL for “Systemic inflammation triggers long-lasting neuroinflammation and accelerates neurodegeneration in a rat model of Parkinson’s disease overexpressing human α-synuclein”. protocols.io https://dx.doi.org/10.17504/protocols.io.j8nlkoj65v5r/v2Version created by Nicola Biagio Mercuri
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: March 18, 2024
Last Modified: May 31, 2024
Collection Integer ID: 96852
Keywords: ASAPCRN
Funders Acknowledgement:
Aligning Science Across Parkinson’s (ASAP)
Grant ID: ASAP-020505
Abstract
Methodological collection for characterization of a dual-hit animal model to assess Parkinson's disease-like symptoms progression
Files
Protocol
Lipopolysaccharide intraperitoneal injection in rats and sickness behavior assessment 
Name
Lipopolysaccharide intraperitoneal injection in rats and sickness behavior assessment 
Version 2
Nicola Biagio Mercuri
Nicola Biagio MercuriFondazione Santa Lucia IRCSS
Protocol
Intracardiac perfusion and rat brain fixation for immunohistochemistry
Name
Intracardiac perfusion and rat brain fixation for immunohistochemistry
Version 1
Nicola Biagio Mercuri
Nicola Biagio MercuriFondazione Santa Lucia IRCSS
Protocol
Immunophenotyping of immune cells by high dimensional flow cytometry
Name
Immunophenotyping of immune cells by high dimensional flow cytometry
Version 1
n.berretta
n.berretta
Protocol
Immunophenotyping of the peripheral blood immune cells by flow cytometry
Name
Immunophenotyping of the peripheral blood immune cells by flow cytometry
Version 1
n.berretta
n.berretta
Protocol
Immunohistochemistry free-floating rat brain cryosections  
Name
Immunohistochemistry free-floating rat brain cryosections  
Version 1
mariangela.massarocenere
mariangela.massarocenere
Protocol
Immunofluorescence free-floating rat brain cryosections
Name
Immunofluorescence free-floating rat brain cryosections
Version 1
Nicola Biagio Mercuri
Nicola Biagio MercuriFondazione Santa Lucia IRCSS
Protocol
Stereology-mediated cell count using StereoInvestigator
Name
ForkStereology-mediated cell count using StereoInvestigator
Version 1
mariangela.massarocenere
mariangela.massarocenere
Protocol
Optical densitometry of tyrosine hydroxylase fibers
Name
Optical densitometry of tyrosine hydroxylase fibers
Version 1
Nicola Biagio Mercuri
Nicola Biagio MercuriFondazione Santa Lucia IRCSS
Protocol
Sholl analysis 
Name
Sholl analysis 
Version 1
Nicola Biagio Mercuri
Nicola Biagio MercuriFondazione Santa Lucia IRCSS
Protocol
Constant Potential Amperometry in vitro
Name
Constant Potential Amperometry in vitro
Version 1
n.berretta
n.berretta
Protocol
Rotarod test 
Name
Rotarod test 
Version 1
n.berretta
n.berretta
Protocol
Open field test 
Name
Open field test 
Version 1
Nicola Biagio Mercuri
Nicola Biagio MercuriFondazione Santa Lucia IRCSS
Protocol
Sucrose preference test 
Name
Sucrose preference test 
Version 1
Nicola Biagio Mercuri
Nicola Biagio MercuriFondazione Santa Lucia IRCSS