May 03, 2019

Public workspaceProtocol for sampling and transport of nose- and thoratsamples in the Fit Futures study V.2

PLOS One
DOI
https://dx.doi.org/10.17504/protocols.io.2j5gcq6
Protocol for sampling and transport of nose- and thoratsamples in the Fit Futures study
  • Dina Stensen1,
  • Anne-Sofie Furberg2,
  • Karina Olsen3,
  • Gunnar Skov Simonsen3,
  • Johanna UE Sollid4,
  • Lars Småbrekke5,
  • Guri Grimnes6,
  • Cristopher Sievert Nielsen7
  • 1University hospital of North Norway;
  • 2Department of Community Medicine, Faculty of Health Sciences, UiT The Arctic University of Norway;
  • 3Department of Microbiology and Infection Control, University Hospital of North Norway;
  • 4Research Group for Host-Microbe Interaction, Department of Medical Biology;
  • 5Department of Pharmacy, Faculty of Health Sciences, UiT The Arctic University of Norway;
  • 6Endocrinology Research Group, Department of Clinical Medicine, Faculty of Health Sciences, UiT The Arctic University of Norway;
  • 7Division of Ageing and Health, Norwegian Institute of Public Health
Dina Stensen
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DOI: https://dx.doi.org/10.17504/protocols.io.2j5gcq6
External link: https://doi.org/10.1371/journal.pone.0218511
Protocol Citation: Dina Stensen, Anne-Sofie Furberg, Karina Olsen, Gunnar Skov Simonsen, Johanna UE Sollid, Lars Småbrekke, Guri Grimnes, Cristopher Sievert Nielsen 2019. Protocol for sampling and transport of nose- and thoratsamples in the Fit Futures study. protocols.io https://dx.doi.org/10.17504/protocols.io.2j5gcq6
Manuscript citation:
Stensen DB, Småbrekke L, Olsen K, Grimnes G, Nielsen CS, Simonsen GS, Sollid JUE, Furberg A (2019) Hormonal contraceptive use and Staphylococcus aureus nasal and throat carriage in a Norwegian youth population. PLoS ONE 14(7): e0218511. doi: 10.1371/journal.pone.0218511
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 03, 2019
Last Modified: May 03, 2019
Protocol Integer ID: 22877
Keywords: other possible riskfactors for staphylococcus aureus carriage, prevalence of staphylococcus aureus, staphylococcus aureus carriage, staphylococcus aureus, thoratsamples in the fit futures study, fit futures study, prevalence, aim of the study
Abstract
The aim of the study is to evaluate prevalence of Staphylococcus aureus in a youth population. This samples will be connected to other possible riskfactors for Staphylococcus aureus carriage.
Materials
MATERIALS
ReagentSterile NaCl 9 mg/ml (0.9%)B.Braun Medical Inc
ReagentSterile rayon-tipped swabPuritan
ReagentTransport medium - Amies Copan
ReagentTongue spatula - wood
ReagentHand disinfectionAntibac
4 Sterile rayon-tipped swabs for each participant
Troubleshooting
Before start
Wash hands with soap and water or use hand disinfection to prevent contamination of the samples. The examiner should use facemask to protect the participant if necessary. If the participant has secreation from the nasal cavity, the participant should blow ones nose before sampling.
Nasal samples
Moisture the rayon-tipped swab in 2-3 drops of sterile NaCl 0,9 mg/ml. There should be two samples from the nasal cavity.
Lead the rayon-tipped swab carefully into the nasal cavity approximately 1-2 cm and rotate with delicate pressure 3 times in both nasal cavity. The same swab should be used for both anterior nares.
Place both swabs in the Coban transport medium.
Label the transport medium with testnumber and nasal swab 1 and 2. Nasal swab 1 is used for detection of bacterial analysis, and swab 2 for microbiome analysis.
Wash hands with soap and water or use hand disinfection after procedure
Throat samples
Moisture the rayon-tipped swab in 2-3 drops of sterile NaCl 0,9 mg/ml. There should be two samples from the throat.
Ask the participant to open his/her mouth. Press the tongue spatula against the tongue and ask the participant to say "aaa". This will simplify the sampling because this will better the view of the tonsil region.
Lead the rayon-tipped swab into the oral cavity and swab with moderate pressure on both tonsil regions (palatine tonsils). If the participants has had a former tonsillectomy/tonsillotomy the swab should be taken from the area of the removed tonsils. Use the same swab on both tonsils.
Place both swabs in the Coban transport medium.
Label the transport medium with testnumber and throat swab 1 and 2. Throat swab 1 is used for detection of bacterial analysis, and swab 2 for microbiome analysis.
Wash hands with soap and water or use hand disinfection after procedure
Transport
The swabs should be placed in a suitable rack. If not picked up immediately, the samples should be placed in refrigerator awating pickup.