This protocol provides a guide for conducting exposures of H. boettgeri specimens to Bd, FV3, or both pathogens, ensuring the welfare of the amphibians and the accuracy of the experiments. Since all FV3 inoculation methods were effective in H. boettgeri, we recommend using the 'water bath' method as it is the least invasive approach. By following these detailed procedures and utilizing the specified materials, researchers can contribute to the advancement of our understanding of amphibian diseases and co-infections, ultimately aiding in their conservation and protection.