Buffer and Chemicals Required
CTAB- 20 g/L
Tris- 12.11 g/L
EDTA- 7.44 g/L
NaCl - 81.89 g/L
2. RNAse A (10mg/ml)- Dissolve in TNE buffer
RNase A can be dissolved at a concentration of 1 to 10 mg/ml in 10 mM Tris-HCl, pH 7.5, 15 mM NaCl, heated to
100 °C for 15 minutes to inactivate contaminating DNases and cooled slowly to room temperature and dispend
into aliquots. Roche recommends subsequent storage at -15 to -25 °C.
3. Proteinase K (20mg/ml) : Dissolve in TE buffer
4. Phenol:Chloroform kit - Stored at 4 °C
5. Isopropanol (room temperature)
7. 70 % Ethanol (Ice-cold)
11.Size Marker: 1 kb DNA ladder
3. Spectrophotometer- Nanodrop
4. Gel Documentation system