Male C57BL/6J mice, aged 6-8 weeks and weighing 19-21g, were purchased from DOSSY EXPERIMENTAL ANIMALS CO. LTD, Chengdu, China. The mice were adaptively raised in an environment with a 12h/12h light/dark cycle, constant room temperature of 26°C, and adequate water and food for one week before the experiment. All experimental procedures followed the Statement for the Use of Animals in Ophthalmic and Vision Research of the Association for Research in Vision and Ophthalmology (ARVO) and were under the supervision of the Institutional Animal Care and Use Committee (IACUC) of Sichuan University (Protocol Number: 20220907001). Before RVO modeling and imaging examination, mice were routinely anesthetized by intraperitoneal injection of 1% sodium pentobarbital, 0.15mL/20g, and pupils were dilated by Mydrin‱ eyedrops (Santen Pharmaceutical Co., LTD., Osaka, Japan). The mice were euthanized by cervical dislocation at the end of the intervention.