Dec 15, 2023
Protocol for "Development and characterization of a non-human primate model of diseeminated synucleinopathy"
- jlanciego1,2,3,
- Alberto J. Rico1,2,3,
- Julia Chocarro Garcia1,2,3,
- Goiaz Ariznabarreta1,2,3,
- Elvira Roda1,2,3,
- Adriana Honrubia1,2,3,
- Patricia Arnaiz1,2,3,
- Almudena Corcho1
- 1CNS Gene Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Pamplona, Spain;
- 2Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas (CiberNed-ISCIII), Madrid, Spain;
- 3Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD, USA
Collection Citation: jlanciego, Alberto J. Rico, Julia Chocarro Garcia, Goiaz Ariznabarreta, Elvira Roda, Adriana Honrubia, Patricia Arnaiz, Almudena Corcho 2023. Protocol for "Development and characterization of a non-human primate model of diseeminated synucleinopathy". protocols.io https://dx.doi.org/10.17504/protocols.io.81wgbxwnylpk/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: December 15, 2023
Last Modified: December 15, 2023
Collection Integer ID: 92381
Keywords: Alpha-synuclein, Lewy body dementia, adeno-associated viral vectors, gene therapy, Parkinson's disease
Funders Acknowledgement:
Aligning Science Across Parkinson's
Grant ID: ASAP-020505
Ministerio de Ciencia e innovación
Grant ID: PID2020-120308RB
Ciberned Intramural Collaborative Projects
Grant ID: PI2020/09
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DISCLAIMER – FOR INFORMATIONAL PURPOSES ONLY; USE AT YOUR OWN RISK
The protocol content here is for informational purposes only and does not constitute legal, medical, clinical, or safety advice, or otherwise; content added to protocols.io is not peer reviewed and may not have undergone a formal approval of any kind. Information presented in this protocol should not substitute for independent professional judgment, advice, diagnosis, or treatment. Any action you take or refrain from taking using or relying upon the information presented here is strictly at your own risk. You agree that neither the Company nor any of the authors, contributors, administrators, or anyone else associated with protocols.io, can be held responsible for your use of the information contained in or linked to this protocol or any of our Sites/Apps and Services.
Abstract
Here we took advantage of a retrogradely-spreading adeno-associated viral vector
serotype 9 coding for the alpha-synuclein A53T mutated gene to induce a
widespread synucleinopathy of cortical and subcortical territories innervating
the putamen. Four weeks post-AAV deliveries animals were sacrificed and a
comprehensive biodistribution study was conducted, comprising the
quantification of neurons expressing alpha-synuclein, rostrocaudal distribution
and their specific location.
This collection is made of (1) study design, (2) experimental animals, (3) viral vector production, (4) stereotaxic surgery for AAV deliveries, (5) necropsy, tissue processing and data analysis, (6) quantification of neurons expressing alpha-synuclein, (7) quantification of the nigrostriatal system
4 literature references included
3 figures included, as follows:
- Figure 1: Viral vector (synapsin)AAV9-SynA53T
- Figure 2: Ventriculography-assisted stereotaxic surgery
- Figure 3: Quantification of alpha-synuclein positive neurons
DISCLAIMER – FOR INFORMATIONAL PURPOSES ONLY; USE AT YOUR OWN RISK
The protocol content here is for informational purposes only and does not constitute legal, medical, clinical, or safety advice, or otherwise; content added to protocols.io is not peer reviewed and may not have undergone a formal approval of any kind. Information presented in this protocol should not substitute for independent professional judgment, advice, diagnosis, or treatment. Any action you take or refrain from taking using or relying upon the information presented here is strictly at your own risk. You agree that neither the Company nor any of the authors, contributors, administrators, or anyone else associated with protocols.io, can be held responsible for your use of the information contained in or linked to this protocol or any of our Sites/Apps and Services.
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Protocol references
Lanciego, J.L., and Vázquez, A. 2012. The basal
ganglia and thalamus of the long-tailed macaque in stereotaxic coordinates. A
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Martin, R.F., and Bowden, D.M. 1996. Template Atlas of the Primate Brain.
University of Washington, Seattle, WA.
Penttinen, A-M., Parkkinen, I., Blom, S., Kopra, J.,
Andressoo, J-A., Pitkänen, K., et al.
2018. Implementation of deep neural networks to count dopamine neurons in
substantia nigra. Eur. J. Neurosci.
48, 2354-2362. doi: 10.1111/ejn.14129
Ruifrok, A.C., and Johnston, D.A. 2011. Quantification
of histochemical staining by color deconvolution. Anal. Quant. Cytol. Histol. 23, 291-299.