Oct 14, 2024

Public workspaceProtocol collection associated with "Endo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons"

  • 1Harvard Medical School;
  • 2Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD 20815, USA
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Collection CitationFrances V Hundley, Miguel A. Gonzalez-Lozano, Harper JW 2024. Protocol collection associated with "Endo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons". protocols.io https://dx.doi.org/10.17504/protocols.io.q26g71o38gwz/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: October 11, 2024
Last Modified: October 14, 2024
Collection Integer ID: 109707
Keywords: ASAPCRN
Funders Acknowledgement:
Aligning Science Across Parkinson's
Grant ID: ASAP-000282
Disclaimer
DISCLAIMER – FOR INFORMATIONAL PURPOSES ONLY; USE AT YOUR OWN RISK

The protocol content here is for informational purposes only and does not constitute legal, medical, clinical, or safety advice, or otherwise; content added to protocols.io is not peer reviewed and may not have undergone a formal approval of any kind. Information presented in this protocol should not substitute for independent professional judgment, advice, diagnosis, or treatment. Any action you take or refrain from taking using or relying upon the information presented here is strictly at your own risk. You agree that neither the Company nor any of the authors, contributors, administrators, or anyone else associated with protocols.io, can be held responsible for your use of the information contained in or linked to this protocol or any of our Sites/Apps and Services.
Abstract
We present a protocol collection for "Endo-IP and Lyso-IP Toolkit for Endolysosomal Profiling of Human Induced Neurons".
DISCLAIMER – FOR INFORMATIONAL PURPOSES ONLY; USE AT YOUR OWN RISK

The protocol content here is for informational purposes only and does not constitute legal, medical, clinical, or safety advice, or otherwise; content added to protocols.io is not peer reviewed and may not have undergone a formal approval of any kind. Information presented in this protocol should not substitute for independent professional judgment, advice, diagnosis, or treatment. Any action you take or refrain from taking using or relying upon the information presented here is strictly at your own risk. You agree that neither the Company nor any of the authors, contributors, administrators, or anyone else associated with protocols.io, can be held responsible for your use of the information contained in or linked to this protocol or any of our Sites/Apps and Services.
Files
Protocol
Icon representing the file Immunofluorescence of endolysosomal markers in human iNeurons
Name
Immunofluorescence of endolysosomal markers in human iNeurons
Version 1
, Harvard Medical School
Frances V HundleyHarvard Medical School
Protocol
Icon representing the file Endo-IP and Lyso-IP in hESCs and iNeurons 
Name
Endo-IP and Lyso-IP in hESCs and iNeurons 
Version 1
, Harvard Medical School
Frances V HundleyHarvard Medical School
Protocol
Icon representing the file SDS-PAGE and immunoblotting to assess whole-cell lysates and Endo-IPs and Lyso-IPs in hESCs and iNeurons
Name
SDS-PAGE and immunoblotting to assess whole-cell lysates and Endo-IPs and Lyso-IPs in hESCs and iNeurons
Version 1
, Harvard Medical School
Frances V HundleyHarvard Medical School