Mar 30, 2023

Public workspaceProtocol collection - "Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism"

  • 1German Center for Neurodegenerative Diseases (DZNE), Tübingen, 72076 Germany
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Collection Citationmichela.deleidi, María José Pérez J., Pascale Baden, Federico Bertoli, Hariam Raji 2023. Protocol collection - "Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism". protocols.io https://dx.doi.org/10.17504/protocols.io.6qpvr4zr3gmk/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: March 30, 2023
Last Modified: May 31, 2024
Collection Integer ID: 79782
Keywords: ASAPCRN
Abstract
Collection of protocols of Deleidi Lab used in the publication: "Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism"
Files
Protocol
Icon representing the file Gcase co-immunoprecipitation
Name
Gcase co-immunoprecipitation
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Single cell dissociation of brain organoids
Name
Single cell dissociation of brain organoids
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Plasmid-reprogramming of human fibroblasts
Name
Plasmid-reprogramming of human fibroblasts
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Sequencing of construct
Name
Sequencing of construct
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Single cell analysis of iPSC-derived midbrain organoids
Name
Single cell analysis of iPSC-derived midbrain organoids
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Complex I activity assay
Name
Complex I activity assay
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Midbrain-like Organoids generation from hiPSCs
Name
Midbrain-like Organoids generation from hiPSCs
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Expansion microscopy
Name
Expansion microscopy
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Soluble and insoluble A-SYN fractionation
Name
Soluble and insoluble A-SYN fractionation
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Mitochondrial complex activity assays
Name
Mitochondrial complex activity assays
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Neuromelanin staining (Fontana-Masson staining)+ TH-DAB staining on midbrain organoids
Name
Neuromelanin staining (Fontana-Masson staining)+ TH-DAB staining on midbrain organoids
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file Differentiation	NPCs	to Dopaminergic/Midbrain Neurons
Name
Differentiation NPCs to Dopaminergic/Midbrain Neurons
Version 1
, ASAP
Federico BertoliASAP
Protocol
Icon representing the file In vitro GCase activity assay (total cell lysate)
Name
In vitro GCase activity assay (total cell lysate)
Version 1
, ASAP
Federico BertoliASAP