Mar 20, 2024

Public workspacePROTEIN INJECTION PROTOCOL

DOI
dx.doi.org/10.17504/protocols.io.dm6gp3738vzp/v1
  • Scott Vermilyea1
  • 1University of Minnesota
Jane Balster
Open access
DOI: dx.doi.org/10.17504/protocols.io.dm6gp3738vzp/v1
Protocol Citation: Scott Vermilyea 2024. PROTEIN INJECTION PROTOCOL. protocols.io https://dx.doi.org/10.17504/protocols.io.dm6gp3738vzp/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 22, 2024
Last Modified: May 31, 2024
Protocol Integer ID: 95761
Keywords: ASAPCRN
Abstract
This protocol details the injection parameters of the protein.
Attachments
Protein Injection Protocol.docx
Protein Injection Pr...
17KB
Materials
  • Needle 28ga
  • Hamilton syringe
  • Constant pressure syringe pump (Harvard Apparatus, Holliston, MA)
Injection Parameters
Injection Parameters
Stereotaxic coordinates:
Intracortical/Intrastriatal (IC/IS): 2.0 mm lateral from the midline, +0.2 mm relative to bregma, and 0.8 and 2.6 mm deep from the dura.
Brainstem: 0.2 mm lateral from the midline, -7.34 mm from bregma, 3.75 mm deep from the dura.

Injectable:
Amount2.5 µg of total protein in a volume of Amount2.5 µL .

Rate: Amount0.1 µL per minute

Needle: 28 ga needle attached via tubing to a Hamilton syringe controlled by a constant pressure syringe pump (Harvard Apparatus, Holliston, MA).