Dissolve pellet in lysis buffer2: 150 mM NaCl, 1% Triton X-100, 50 mM TrisHCl (pH8), 5 mM EDTA. Supplement lysis buffer with cOmplete Protease Inhibitor Coctail (Roche) (1 tablet per 10 ml of solution) and Benzonase nuclease (Sigma) immediately before extraction to prevent protein degradation and to decrease viscosity of extract, respectively.