ONT primer preparation has two specific aspects that are unique when comparing to Sanger sequencing protocols. The first is that each primer needs to be "tagged" - a unique ~10-15bp sequence is added to the 5' end of the forward and reverse primers. Secondarily, these tagged primers need to be "multiplexed" - meaning that each individual specimen has a unique combination of forward-tagged primer and reverse-tagged primer. These tags allow the DNA amplicons for all of the specimens to be "pooled" or mixed together for sequencing, and then to be "demultiplexed" or sorted back out, allowing the resulting sequences to be associated with the individual specimens they originated from.
It is possible to perform ONT sequencing without the tags, but you would not be able to associate the sequences with any individual specimens. Ex - If you had ten closely related Russula specimens in your sequencing run, you would be able to document sequences of all ten of the species, but you would have a difficult time associating the sequences with the individual specimens/observations they originated from. They would be sequences without faces. This may be common if you are examining the community ecology or environmental DNA of a particular location, but this result would not be ideal for most DNA barcoding goals involving specimens. Thus, if you are running 960 specimens on a Flongle flowcell, you need to have 960 unique primer combinations for the sequencing run.
The easiest way to accomplish this is to have a single unique forward primer tag for each plate you are including, combined with 96 unique reverse primers for the plate. If you are including five plates, you would have five unique forward primers (a different one for each plate) combined with a standard set of 96 reverse primers. This results in 960 unique tags for each of the 960 specimens that are being barcoded.
Note: This protocol utilized a primer plate of 96 different reverse primers. In the future I plan on ordering the primer plate of 96 unique primers for the forward ITS1F primers. This will allow ad-hoc combinations of a different ONT-tagged reverse primer to extend into the LSU region as needed.