The following materials list contains those consumables+quantities used specifically at the IMR to complete the present protocol for 380 amplicons (4 plates) on one MiSeq run.
Phusion Plus XL pack (2000 U = 4000 rxns)
Thermo dNTP mix (10 mM x 10 mL = 20000 rxns)
Separate Illumina fusion primer sets (4000 uL/ea. @ 1 uM = 1000 rxns)
…or Combined UDI F+R Illumina fusion primer sets (4000 uL/pair @ 1 uM = 1000 rxns/pair)
PCR microplates 96-well Bio-Rad
PCR microplates sealing film Bio-Rad
PCR microplates 96-well Bio-Rad
PCR microplates sealing film Bio-Rad
E-gels or Coastal Genomics analytical gels
Initial Clean-up and Normalization
PCR microplates sealing film Bio-Rad
Charm Normalization 96-well kit
Final Pooling and Clean-up
Illumina SPB (or AMPure or Pronex beads)
PCR microplates 96-well Bio-Rad
MiSeq Reagent Kit v3 600 cycle
Quant-iT 1X HS dsDNA Kit (1000 samples)
Various manual pipette tips