In order for bacteria to be successfully cultured, they must be grown in the appropriate media. LB, also known as Lysogeny broth (also known as Luria broth, Lennox broth, or Luria-Bertani medium.), is a nutrient rich broth that is a standard for culturing Escherichia coli, as it allows for quick growth and high yields. Furthermore, addition of agar to LB broth creates a gel for bacteria to grow upon, and is therefore used for plating bacterial cultures on petri dishes for single colony isolation.
This procedure is to be used for the preparation of LB media and LB plates
The formulations generally differ in the amount of sodium chloride, thus providing selection of the appropriate osmotic conditions for the particular bacterial strain and desired culture conditions. The low salt formulations, Lennox and Luria, are ideal for cultures requiring salt-sensitive antibiotics.
This SOP includes instructions to make either 1litre or 120ml LB broth.