Oct 17, 2023
Preparing Cholesterol for use in NGM
- 1Imperial College London, MRC Laboratory of Medical Sciences
Protocol Citation: Bonnie Evans 2023. Preparing Cholesterol for use in NGM. protocols.io https://dx.doi.org/10.17504/protocols.io.36wgq3oe5lk5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 09, 2023
Last Modified: October 17, 2023
Protocol Integer ID: 89018
Abstract
C. elegans requires cholesterol to progress through the four larval stages.
Materials
CHOLESTEROL-PEG 600 Sigma- C1145-250MG (stored at -20 degrees)
Ethanol absolute (Stored in flammables cupboard)
Prepare 5 mg/ml in Ethanol by resuspending the whole 250mg vial in 50 ml ~100% ethanol.
Filter sterilise using vacuum pump.
Store at 4 degrees protected from the light