A | B | |
Components | Amount in μL for 1x concentration of PCR Master mix | |
PCR grade H2O | 8.8 | |
dNTPs mix (25 mM) | 0.6 | |
10x Thermopol Buffer | 2 | |
MgSO4 (100mM) | 0.6 | |
Cellular reagents (Freshly prepared and heat treated) | 3 | |
20% (w/v) Trehalose stock solution | 4.5 | |
Bromophenol Blue 0.25% (w/v)Azorubine 0.25% (w/v) | 0.5 | |
20 |