A | B | |
Chemical | Amount needed in (g) for 1 M stock | |
Tris | 1.211 | |
KCL | 0.74 | |
NH(SO4)2 | 1.30 | |
MgSO4 | 1.20 |
A | B | |
Chemical stock | Amount required in uL to make a 1000 mL of 10x PCR Buffer | |
1M KCL | 100 | |
1M (NH)2SO4 | 100 | |
1M Tris-HCL pH8.8 @ 25c | 200 | |
1M MgSO4 | 20 | |
Triton x100 | 10 |