FSW: Filtered seawater (0.22 µm)
·Symbiodiniaceae freshly isolated as detailed in Bouwmeester et al. (2022)
(Reference: Bouwmeester J, Daly J, Quinn M, Hagedorn M (2022) Isolation of Symbiodiniaceae from scleractinian coral hosts suitable for cryopreservation and laser-warming. Protocols.io, doi.org/10.17504/protocols.io.b4e3qtgn) ·Filtered seawater (FSW), freshly collected and filtered each day
Chemicals and Nanoparticles
·Trehalose (Pfanstiehl Inc. Waukehan, IL, USA, CAS # 6138-23-4)
Note that this chemical cannot be replaced by a cheaper version as it needs to be of high grade to be tolerated by the algal symbionts
·Alginic acid sodium salt (Sigma-Aldrich, St. Louis, MO, USA, CAS # 9005-38-3)
·Calcium chloride (Sigma-Aldrich, St. Louis, MO, USA, CAS # 10043-52-4)
·Gold nanorod nanoparticles with PEG surface and peak absorbance at 1064 nm, custom-made (Nanocomposix, San Diego, CA, USA)
Glassware and Consumables
·250 mL glass beaker (e.g., Pyrex No 1000)
·Extra fine tea strainer to fit into 250 mL beaker (e.g., Yoassi, ASIN # B01LQ7NQTW)
·20-µL cell strainer basket (Pluriselect, Leipzig, Germany, SKU 43-50020-03)
·15-mL conical Falcon tubes
·Polyresin foam, 6 mm thick (Ben Franklin Craft Store, Hawaii)
·Acetate transparency film (Avatar AT1000 Transparency Film)
·Magnetic stir plate (n=3)
·Centrifuge for 1.5-mL Eppendorf tubes
·Block heater with aluminium blocks for 1.5 mL and 15 mL tubes (e.g., Isotemp 125D, Fisher Scientific)
·Microbalance for weighing chemicals
·Hemacytometer (Hausser Scientific, Horsham, PA, USA, #02-671-51B)
·15-mL Dounce tissue grinder (Wheaton, DWK Life Sciences, Millville, NJ, USA) – use the loose pestle
·Ultrasonic cleaner for sonicating gold nanoparticles (e.g., Co-Z 2L Professional Ultrasonic Cleaner, ASIN # B075FTFD3R)
·Metal ruler or similar object to be used as guideline for cutting through the foam craft sheets with a cutter knife
·Optional: Chemical-friendly microwave