Jun 26, 2024

Public workspacePreparation of PEI for primary coating of MaxOne (neuronal cultures)

DOI
dx.doi.org/10.17504/protocols.io.j8nlk8dy6l5r/v1
  • 1Weizmann Institute
Yonatan Katz
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DOI: dx.doi.org/10.17504/protocols.io.j8nlk8dy6l5r/v1
Protocol Citation: Yonatan Katz 2024. Preparation of PEI for primary coating of MaxOne (neuronal cultures). protocols.io https://dx.doi.org/10.17504/protocols.io.j8nlk8dy6l5r/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: June 25, 2024
Last Modified: June 26, 2024
Protocol Integer ID: 102349
Abstract
Polyethyleneimine (PEI) coating to enhance cell adhesion on MaxOne chips. PEI coating is followed by Laminin coating.
Protocol materials
ReagentPierce™ 20X Borate BufferThermo FisherCatalog #28341
ReagentPoly(ethyleneimine) solutionMerck MilliporeSigma (Sigma-Aldrich)Catalog #P3143
Materials
Materials
ReagentPierce™ 20X Borate BufferThermo FisherCatalog #28341
ReagentPoly(ethyleneimine) solutionMerck MilliporeSigma (Sigma-Aldrich)Catalog #P3143

0.07% PEI solution preparation - Primary coating
0.07% PEI solution preparation - Primary coating
30m
30m
Prepare 100 mL of 1X borate buffer:
Amount5 mL 20X borate buffer
Amount95 mL sterile deionized water

7% PEI stock solution:
Amount1.4 mL 50% = PEI solution directly into a sterile beaker.
Amount8.6 mL 1X borate buffer into the sterile beaker.

Mix Duration00:30:00 using a magnetic stirrer until fully dissolved
(can be stored in 0.5 mL aliquots at -20°C for 1 month).
Where is it sterilized? -

30m
final 0.07% PEI solution
Amount0.5 mL 7% PEI solution in
Amount49.5 mL 1X borate buffer fresh

filter through a 0.22 μm filter unit before use!