Mar 27, 2025
Preparation of Lipopolysaccharide for Intraperitoneal Injection
- Adrien Stanley1
- 1Allen Institute for Neural Dynamics
- Allen Institute for Neural Dynamics
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Abstract
This protocol describes the steps for preparing 20X stock and experimental solution of Lipopolysaccharide (LPS) intended for intraperitoneal injections in mice at a concentration of 0.25 mg/mL.
Prepare 20X Stock Solution
Prepare 20X Stock Solution
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Upon arrival of 5 mg Lipopolysaccharide (LPS) powder, in a fume hood dissolve in 1 mL of pharmaceutical grade sterile saline (0.9%) to make a 5 mg/mL stock solution.
Note
Note: If stock solution isn’t prepared upon arrival, store LPS powder at .
Note: If stock solution isn’t prepared upon arrival, store LPS powder at 4 °C .
Attach 23-Gauge needle to 1 mL syringe.
Swab surface of sterile saline bottle septum with a 70% ethanol swab.
Withdraw volume.
Eject saline in syringe into the bottle containing LPS powder.
Mix the contents for ~ by inverting and shaking the bottle to ensure all powder on the bottle's interior surface is dissolved.
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Pipette aliquots of stock solution in low protein binding tubes and store at .
Remove label from the empty LPS bottle and discard bottle in biohazard waste.
Place label on clipboard that documents used chemicals in the lab.
Prepare 0.5 mg/mL LPS Working Solution for IP Injection
Prepare 0.5 mg/mL LPS Working Solution for IP Injection
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At most before the injection on the experimental day, dilute stock by 20X (to final concentration).
Rapidly thaw stock aliquot by holding fingers to the wall of the tube for about .
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Add of saline and mix by inverting the tube or by vortexing.
Leftover LPS should be discarded in a biohazard waste container.