Oct 11, 2022
Version 2
Preparation of frozen nuclei for single-nucleus RNA seq V.2
- 1Karolinska Institutet
Protocol Citation: Kimberly Siletti, Sten Linnarsson 2022. Preparation of frozen nuclei for single-nucleus RNA seq. protocols.io https://dx.doi.org/10.17504/protocols.io.eq2lyrdpgx9k/v2Version created by Kimberly Siletti
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 11, 2022
Last Modified: October 11, 2022
Protocol Integer ID: 71166
Abstract
This protocol describes our preparation of nuclei for single-nucleus RNA sequencing on the 10X Genomics Chromium system, starting from FAC-sorted frozen nuclei.
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