| Reagent ID | Formula | Name | Recommended Stock Preparation |
| 1 | Na2SO4 | Sodium sulphate | 8.5g in 50mL water (X100) |
| 2 | Na3C6H5O7.2H2O | Tri-sodium citrate | 3.6g in 50mL water (X100) |
| 3 | C4H7N3O | Creatinine | 4.05g in 50mL water (X100) do not chill |
| 4 | CH4N2O | Urea | (15g per 100mL) 22.5g in 150mL water (X10) made fresh on the day of combining reagents. Endothermic, warm to 37C to prevent cooling the mixture upon addition and causing other salts to precipitate Calculate how much required on the day. |
| 5 | KCl | Potassium chloride | 11.54g in 50mL water (X100) |
| 6 | NaCl | Sodium chloride | 8.78g in 50mL water (X100) |
| 7 | CaCl2 | Calcium chloride anhydrous | 0.925g in 50mL water (X100) caution - alkali |
| 8 | NH4Cl | Ammonium chloride | 6.33g in 50mL water (X100) |
| 9 | K2C2O4.H2O | Potassium oxalate | 2 steps: dissolve 1.75g in 50mL water (X1000) then transfer 5mL to a new tube and make up to 50mL with water for X100 |
| 10 | MgSO4.7H2O | Magnesium sulphate | 5.41g in 50mL water (X100) |
| 11 | NaH2PO4.2H2O | Sodium dihydrogen orthophosphate dihydrate | 14.56g in 50mL water (X100) |
| 12 | Na2HPO4.2H2O | Sodium phosphate monobasic dihydrate | 4.155g in 50mL water (X100) |
| 13 | | Bacteriological Peptone | (2g per 100mL) 9g in 450mL water in a glass bottle (X10) and autoclaved. |
| 14 | | Yeast Extract | 0.18g in 100mL water (X100) autoclave |