Jun 10, 2022
Preparation of 1M calcium chloride solution (CaCl2)
- 1Université Paul Sabatier (Toulouse III)
- Perez-Escudero Lab
Protocol Citation: Alfonso Pérez Escudero, Alid Al-Asmar, gabrielmadirolas 2022. Preparation of 1M calcium chloride solution (CaCl2). protocols.io https://dx.doi.org/10.17504/protocols.io.36wgq7mokvk5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: May 03, 2022
Last Modified: June 10, 2022
Protocol Integer ID: 61890
Keywords: ngm, worm, c.elegans, c elegans, elegans, caenorhabditis, media, medium, calcium chloride
Abstract
Original source of the protocol: WormBook Methods http://www.wormbook.org/chapters/www_strainmaintain/strainmaintain.html
This protocol is for making 1M calcium chloride solution, which is used in the preparation of worm media, like NGM (Nematode Growth Medium).
Materials
Calcium chloride:Calcium Chloride DihydrateSigmaCatalog #C7902-500G
Filter: Rapid-Flow Nalgene 0.2µm aPES membrane, ref: 564-0020
Vaccum pump: Fisher Brand, ref: FB70155
Weigh 7.4 g ±0.05 of calcium chloride dihydrate (CaCl2·2H2O).
Dissolve it in ~40 mL of milliQ water in a 100mL measuring cylinder.
Add a clean stirring magnet, and leave it on a stirrer until all crystals are dissolved.
Remove the stirring magnet: To prevent touching the solution, use another magnet from the outside of the measuring cylinder to slide the stirring magnet out of the cylinder.
Fill up to 50 mL .
Filter-sterilize.
Store at 4°C.
We usually use it without problem for up to at least a year after preparation.