Nov 12, 2023
Preparation and Administration of Systemic 4-AP
- 1University of California, San Diego
Protocol Citation: Leonardo A Parra-Rivas 2023. Preparation and Administration of Systemic 4-AP. protocols.io https://dx.doi.org/10.17504/protocols.io.5qpvo38dxv4o/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 12, 2023
Last Modified: November 12, 2023
Protocol Integer ID: 90816
Abstract
Preparation and Administration of Systemic 4-AP
4-Aminopyridine was obtained from Sigma-Aldrich (Cat #A78403), and a stock solution was
prepared in saline. Mice (8 weeks old) were given intraperitoneal injections of
soluble 4-AP at a low dose of 2 mg/Kg or equivalent saline.
Animals were monitored every 20 min and presented no sign of convulsive seizure behavior.
After 3 hrs, animals were anesthetized with isoflurane (VetOne Cat#502017),
followed by cervical dislocation. Brains were rapidly removed and drop-fixed in
4% PFA solution for immunohistochemistry or placed on ice for biochemical
assays.