Feb 06, 2023

Public workspace Potassium phosphate buffer (1.0 M, pH 6.2) V.1

This protocol is a draft, published without a DOI.
  • 1University of Oslo
Andreas Sagen
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Protocol Citation: Andreas Sagen 2023. Potassium phosphate buffer (1.0 M, pH 6.2). protocols.io https://protocols.io/view/potassium-phosphate-buffer-1-0-m-ph-6-2-cnujveun
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Other
We attempted this protocol but could not get it to work in our workspace
Created: February 04, 2023
Last Modified: February 06, 2023
Protocol Integer ID: 76395
Keywords: potassium phosphate buffer, buffer, protein
Abstract
Potassium phosphate buffers are buffers with a buffering capacity between 5.8 and 8.0, where it is possible to adjust the pH and buffer strength with different ratios of Sodium phosphate monobasic and Sodium phosphate dibasic.
Materials
Scale
Magnetic stirrer
Filtering device
Protocol materials
ReagentSodium phosphate dibasicMerck MilliporeSigma (Sigma-Aldrich)Catalog #S0876
Step 2
ReagentSodium phosphate monobasicMerck MilliporeSigma (Sigma-Aldrich)Catalog #S0751
Step 2
500 mL Phosphate buffer (1.0 M, pH 6.2)
500 mL Phosphate buffer (1.0 M, pH 6.2)
5m
5m
Fill a sterile Amount500 mL bottle with Amount400 mL distilled water
Measure Amount12.035 g Sodium phosphate dibasic and Amount49.818 g Sodium phosphate monobasic

Materials:
ReagentSodium phosphate dibasicSigma AldrichCatalog #S0876
ReagentSodium phosphate monobasicSigma AldrichCatalog #S0751
Add dry ingredients and mix for Duration00:05:00
5m
Fill to Amount500 mL with distilled water
Filter sterilize through a 0.2 µm filter and store refrigerated (Temperature4 °C )