Feb 21, 2023

Public workspacePotassium phosphate buffer (0.9 M, pH 7.7) V.2

This protocol is a draft, published without a DOI.
  • 1University of Oslo
Andreas Sagen
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Protocol Citation: Andreas Sagen 2023. Potassium phosphate buffer (0.9 M, pH 7.7). protocols.io https://protocols.io/view/potassium-phosphate-buffer-0-9-m-ph-7-7-cprwvm7eVersion created by Andreas Sagen
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: February 20, 2023
Last Modified: February 21, 2023
Protocol Integer ID: 77334
Keywords: potassium phosphate buffer, buffer, protein
Abstract
Potassium phosphate buffers are buffers with a buffering capacity between 5.8 and 8.0, where it is possible to adjust the pH and buffer strength with different ratios of Sodium phosphate monobasic and Sodium phosphate dibasic.
Materials
Scale
Magnetic stirrer
Filtering device
Protocol materials
ReagentPotassium phosphate dibasicMerck MilliporeSigma (Sigma-Aldrich)Catalog #P3786
Step 2
ReagentPotassium phosphate monobasicMerck MilliporeSigma (Sigma-Aldrich)Catalog #P5379
Step 2
500 mL Phosphate buffer (0.9 M, pH 7.7)
500 mL Phosphate buffer (0.9 M, pH 7.7)
5m
5m
Fill a sterile Amount500 mL bottle with Amount400 mL distilled water
Measure Amount62.705 g Potassium phosphate dibasic and Amount11.568 g Potassium phosphate monobasic

Materials:
ReagentPotassium phosphate dibasicSigma AldrichCatalog #P3786
ReagentPotassium phosphate monobasicSigma AldrichCatalog #P5379
Add dry ingredients and mix for Duration00:05:00
5m
Fill to Amount500 mL with distilled water
Filter sterilize through a 0.2 µm filter and store refrigerated (Temperature4 °C )