Oct 11, 2022

Public workspacePost-Surgical Dissection of Fallopian Tubes

DOI
dx.doi.org/10.17504/protocols.io.14egn75eqv5d/v1
  • 1University of Pennsylvania;
  • 2Gift of Life Donor Program
Stephen Fisher
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DOI: dx.doi.org/10.17504/protocols.io.14egn75eqv5d/v1
Protocol Citation: Stephen Fisher, Marielena Grijalva, Rong Guo, Sarah A Johnston, Hieu Nguyen, John Renz, Jean G Rosario, Steven Rudich, Brian Gregory, Junhyong Kim, Kate O'Neill 2022. Post-Surgical Dissection of Fallopian Tubes. protocols.io https://dx.doi.org/10.17504/protocols.io.14egn75eqv5d/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 05, 2022
Last Modified: October 11, 2022
Protocol Integer ID: 68275
Funders Acknowledgement:
NIH
Grant ID: U54HD104392
Abstract
This protocol describes dissection of the Fallopian tubes in preparation for 10X Visium, 10X Multiomics, pathology review, and biobanking. The Fallopian tubes have an inner mucosa surrounded by a muscular layer that is coated by serosa. When sectioning the Fallopian tube the muscule layer may retract or protrude and distort orientation. This can be addressed by using a stabilizing hand or dissection pins.
Materials
  • Ice
  • Clean cutting surface
  • Disposable scalpels
  • Kimwipes
  • Disposble rulers (with centimeter measurements)
  • Ice bucket
  • Biohazard bags and container
  • ReagentNuclease-free Water
  • ReagentDMEM/F-12Thermo FisherCatalog #11320033
  • ReagentRPMI 1640 MediumThermo FisherCatalog #11875085
  • ReagentMACS® Tissue Storage SolutionMiltenyi BiotecCatalog #130-100-008
  • ReagentDPBS (10X), no calcium, no magnesium Thermo Fisher ScientificCatalog #14200075 - dilute to 1X with nuclease-free water
  • ReagentMarking dyeCancer DiagnosticsCatalog #03000P
Equipment
VWR® Pour-Boat Weighing Dishes
NAME
flat-bottomed dish
TYPE
VWR International
BRAND
10803-166
SKU



Before start
Dilute phosphate buffered saline (Gibco; 14200-075) to 1X with nuclease-free water.
After removing uterus and Fallopian tubes from ice, dry each Fallopian tube and use marking dye to label the superior edge (12 o’clock), inferior edge (6 o’clock), anterior surface (3 o’clock) and posterior surface (9 o’clock).
Measure the Fallopian tube from cornua to fimbriated end.

Measurement of Fallopian Tube. Left Fallopian tube is displayed from isthmus (left) to fimbriated end (right). Black line made with marking ink at 3 o’clock and red line made at 6 o’clock where the Fallopian tube attaches to the mesosalpinx.

Using a disposable scalpel, divide one Fallopian tube at the cornua and excise mesosalpinx.
Weigh Fallopian tube in a disposable weigh boat on ice.
Place on ice the uterus with the remaining contralateral Fallopian tube.
Remove the first Fallopian tube from ice and place on a cutting surface.
Divide Fallopian tube into four sections: isthmus, ampulla, infundibulum, and fimbriae.
Working one section at a time, slice tissue into four sections.
Using disposable weigh boats, weigh each tissue piece.
Tissue can be processed with protocols OCT-Embedded Tissue Preparation, Tissue Fixation Preparation, or Snap-Frozen Tissue Preparation depending on the desired downstream processing.
Remove the uterus with the remaining contralateral Fallopian tube from ice and repeat above steps.