Oct 18, 2023
Polysomnography
- 1Vall d'Hebron Research Institute
Protocol Citation: Núria Peñuelas, Ariadna Laguna, Miquel Vila 2023. Polysomnography. protocols.io https://dx.doi.org/10.17504/protocols.io.rm7vzx77xgx1/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 18, 2023
Last Modified: November 17, 2023
Protocol Integer ID: 89550
Abstract
Polysomnography in mice
Anesthetize the animals (ketamine and xylazine,100/10 mg/kg. i.p.) for polysomnography.
Screw 3 electrodes to skull above parietal and frontal cortex and cerebellum as the reference for electroencephalogram (EEG).
Slip 2 wire electrodes between neck muscles for electromyogram (EMG).
Braze thebleads to a miniature plug (Plastics One, Bilaney, Germany), cemented to skull using acrylic cement (Superbond, C&B Sun Medical) and coated using dental cement (Paladur,Heraeus Kulzer).
For pain caring, administer a subcutaneous injection of carprofen (5 mg/kg).
Once recovered, house mice in individual barrels under 12h light/dark cycle (light-on 7 a.m.) and constant temperature, tethered to the acquisition system and record 1 week out of 3 over 12 consecutive months until natural death.
Based on amplified/digitized unipolar EEG and bipolar EMG signals, visually score vigilance states using a 5s sliding window frame and assign them as Waking (W), Slow Wave Sleep (SWS) and Paradoxical Sleep (PS) to produce hypnograms and state quantifications (Sleepscore, Viewpoint).