Jun 09, 2023
Pole Test to assess motor coordination in parkinsonian mice
- natalia.lopezgonzalezdelrey1,
- Zachary Gaertner1
- 1Northwestern University, Feinberg School of Medicine, Neurology Department
Protocol Citation: natalia.lopezgonzalezdelrey, Zachary Gaertner 2023. Pole Test to assess motor coordination in parkinsonian mice. protocols.io https://dx.doi.org/10.17504/protocols.io.dm6gp3ed8vzp/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 09, 2023
Last Modified: May 31, 2024
Protocol Integer ID: 83161
Keywords: ASAPCRN, behavior protocol, parkinsonian behavior, pole test
Funders Acknowledgement:
Aligning Science Across Parkinson's [ASAP-020600] through the Michael J. Fox Foundation for Parkinson's Research (MJFF)
Grant ID: ASAP-020600
Abstract
The pole test evaluates the motor coordination ability of a mouse by having them grasp a pole and then descend to its home cage.
Materials
16in tall, 1⁄2in diameter wooden pole coated in tape to allow gripping
Pole Test
Pole Test
1d
1d
Total duration: 1 day
Three consecutive trials per animals are run.
1d
Protocol
Protocol
1h
1h
Place the animal cage in the testing room to allow the animals to acclimate. 01:00:00
1h
Mice are placed with their head oriented upward at the top of a 16in tall, 1⁄2in diameter wooden pole coated in tape to allow gripping. The animals normally orient themselves downward and then descend the length of the pole in order to return to their home cage.
5m
Return the mouse to its cage.
1m
Analysis
Analysis
The time required for the animals to orient themselves facing in a downward direction (time to T turn) and to descend to the base of the pole (total time) is recorded.
Mean times per mouse are compared across groups.