Apr 04, 2024
Pole Test
- Sabina Marciano1,
- Tae-In Kam2,
- Roberta Marongiu1,
- Ted Dawson2
- 1Weill Cornell Medicine;
- 2Johns Hopkins University
Protocol Citation: Sabina Marciano, Tae-In Kam, Roberta Marongiu, Ted Dawson 2024. Pole Test. protocols.io https://dx.doi.org/10.17504/protocols.io.5jyl8938rv2w/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 19, 2022
Last Modified: May 31, 2024
Protocol Integer ID: 68928
Keywords: behavior, ASAPCRN
Funders Acknowledgement:
Aligning Science Across Parkinson's
Grant ID: 020608
Abstract
This behavior test is used to assess motor coordination in mice.
Guidelines
Main outcome measures: average time to turn, average time to reach the bottom
Materials
Metal rod pole wrapped in bandage gauze (9mm in diameter, 75cm in height)
Place the mouse on the vertical pole with their head oriented upwards 7.5cm from the top of the pole.
During day 1, gently guide the mouse to turn around and go to the bottom of the pole. They are trained 3 times.
On day 2, they are trained 3 times again without guidance.
On day 3, the test day, mice are again placed on the pole 3 times.
Record the time to reorient to face down and the time to reach the bottom of the pole during each of the 3 trials.
Protocol references
Based on protocol used in:
Kim S, Kwon SH, Kam TI, Panicker N, Karuppagounder SS, Lee S, Lee JH, Kim WR, Kook M, Foss CA, Shen C, Lee H, Kulkarni S, Pasricha PJ, Lee G, Pomper MG, Dawson VL, Dawson TM, Ko HS. Transneuronal Propagation of Pathologic α-Synuclein from the Gut to the Brain Models Parkinson's Disease. Neuron. 2019 Aug 21;103(4):627-641.e7. doi: 10.1016/j.neuron.2019.05.035. Epub 2019 Jun 26. PMID: 31255487; PMCID: PMC6706297.