Jun 03, 2020
Phosphate Buffered Saline (PBS)
- 1University of Manitoba
Protocol Citation: Neilier Junior 2020. Phosphate Buffered Saline (PBS). protocols.io https://dx.doi.org/10.17504/protocols.io.bfypjpvn
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 05, 2020
Last Modified: June 03, 2020
Protocol Integer ID: 36591
Keywords: pH, Henderson-Hasselback, Biochemistry, Molecular Biology,
Abstract
A buffer solution has the function of resisting changes in pH even when adding powerful acids or bases. However, in the physiological environment the buffered system also provides cofactors for enzymatic reactions, critical salts and even essential nutrients for cells and tissues. Therefore, when trying to reproduce biological conditions in vitro, we must make the appropriate choice of the buffer. After all, it will provide the appropriate medium in which reactions will occur.
Materials
- Distilled Water
- pH Meter (sensitive)
- NaCl
- Potassium Phosphate Buffer
- Na2HPO4
- KH2PO4
Safety warnings
Wear personal protective equipment: gloves, lab coat and mask.
Before start
Organize your workspace.
Make sure all solutions and equipment are available.
Phosphate Buffered Saline (PBS)
Phosphate Buffered Saline (PBS)
Note
7.4
150 mM NaCl
10 mM Potassium Phosphate buffer
Prepare 1 L PBS by dissolving 8.7 g NaCl ,1.82 g K2HPO4 • 3H2O and 0.23 g KH2PO4 in 1 L distilled water .
A variation of PBS can also be prepared as follows:
137 millimolar (mM) NaCl
2.7 millimolar (mM) KCl
10 millimolar (mM) Na2HPO4
1.76 millimolar (mM) KH2PO4
Adjust the pH before use.