Oct 01, 2019

Public workspacePhage lambda genome concatemerization

DOI
dx.doi.org/10.17504/protocols.io.7k3hkyn
  • 1Wageningen University
  • iGEM Wageningen 2019
Marijn Ceelen
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DOI: dx.doi.org/10.17504/protocols.io.7k3hkyn
Protocol Citation: Marijn Ceelen 2019. Phage lambda genome concatemerization. protocols.io https://dx.doi.org/10.17504/protocols.io.7k3hkyn
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: September 23, 2019
Last Modified: October 01, 2019
Protocol Integer ID: 28027
Abstract
This protocol is used to create concatemers of phage genomes. This is done by ligating phage lambda genomes using the overhangs at the cos site with T4 ligase.
Add the following ingredients in a microcentrifuge tube.
Ingredient Amount 
Ligation buffer 10X 2 µL 
DNA  6 µg 
PEG 4000 (40% W/W) 2.5 µL 
T4 DNA ligase 1 µ L (400 Weiss units) 
Water To 20 µL 
Incubate at Temperature15 °C overnight