Sep 19, 2024
Version 1
Perturb-FISHv3 V.1
- 1broad institute
Protocol Citation: loic binan, Sami Farhi, Brian Cleary 2024. Perturb-FISHv3. protocols.io https://dx.doi.org/10.17504/protocols.io.6qpvr356bvmk/v1Version created by Data Wranglers SSPsyGene
Manuscript citation:
Simultaneous CRISPR screening and spatial transcriptomics reveals intracellular, intercellular, and functional transcriptional circuits
Loϊc Binan, Serwah Danquah, Vera Valakh, Brooke Simonton, Jon Bezney, View ORCID ProfileRalda Nehme, Brian Cleary, Samouil L Farhi
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 23, 2024
Last Modified: September 19, 2024
Protocol Integer ID: 103529
Funders Acknowledgement:
National Institute Of Mental Health
Grant ID: U24MH132628
National Institute Of Mental Health
Grant ID: R01MH128366
Abstract
This is the 07-21-2024 update version to this protocol protocol describes how the Sami Farhi lab, https://www.broadinstitute.org/bios/sami-farhi, carries out Perturb-FISH and was provided by Loïc Binan, https://sites.broadinstitute.org/opp/people/lo%C3%AFc-binan. See the author's paper for a wider context of this protocol: https://doi.org/10.1101/2023.11.30.569494
Note: This protocol is being connected to part of the NIMH-initiated consortium SSPsyGene: https://sspsygene.ucsc.edu/, SSPsyGene has the aim to functionally characterize the contribution of multiple genes to neurodevelopmental and psychiatric disorders (NPD) resulting in a comprehensive phenotypic catalog.
Attachments
Image Attribution
Image from Fig1 of https://doi.org/10.1101/2023.11.30.569494