BL21(DE3) competent cells
2XYT + agar plates with carbenicillin
Sterile glass plating beads
Sterile, nutrient-rich liquid media (for transformation outgrowth step)
15 mL SuperBroth, autoclaved in 100 mL flask (for starter culture)
1 L SuperBroth, autoclaved in 2 L flask
Glass pipettes (5, 10, 25 mL), autoclaved
10 mM CAPS pH 11.2, 150 mM NaCl, 20% sucrose, sterile filtered, pre-chilled
10 mM CAPS pH 11.2, 150 mM NaCl, sterile filtered, pre-chilled
10 mM CAPS pH 11.2, sterile filtered pre-chilled
5 M NaCl, sterile filtered
Eppendorf tubes, autoclaved
Beckman Coulter polycarbonate centrifuge tubes (part #363070, ~30 mL vol)
Nalgene 250 mL polycarbonate centrifuge bottles