Oct 28, 2024

Public workspacePELSA protocol V.2

DOI
dx.doi.org/10.17504/protocols.io.q26g717x1gwz/v2
  • 1Dalian Institute of Chemical Physics, Chinese Academy of Sciences;
  • 2European Molecular Biology Laboratory
Kejia Li
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DOI: dx.doi.org/10.17504/protocols.io.q26g717x1gwz/v2
Protocol Citation: Kejia Li, Mingliang Ye 2024. PELSA protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.q26g717x1gwz/v2Version created by Kejia Li
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 01, 2024
Last Modified: October 28, 2024
Protocol Integer ID: 110998
Keywords: functional proteomics, drug target discovery, ligand-protein interactions, proteome-wide profiling
Funders Acknowledgement:
National Key Research and Development Program of China
Grant ID: 2021YFA1302601
the National Natural Science Foundation of China
Grant ID: 92153302
National Natural Science Foundation of China
Grant ID: 22437007
Abstract
This protocol details a step-by-step procedure of PELSA method, a modification-free approach that achieves unprecedented sensitivity in proteome-wide target identification and binding-region determination. Following this procedure, one can determine the binding proteins, binding regions, and binding affinities of the analyte ligand.
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PELSA_protocol.pdf
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