Jul 06, 2022
Version 1
Peanut seed sterilization and germination V.1
This protocol is a draft, published without a DOI.
- Ziliang Luo1,
- Liping Wang1
- 1University of Florida
Protocol Citation: Ziliang Luo, Liping Wang 2022. Peanut seed sterilization and germination. protocols.io https://protocols.io/view/peanut-seed-sterilization-and-germination-ccttswnn
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: July 06, 2022
Last Modified: July 06, 2022
Protocol Integer ID: 66131
Abstract
Treatments of peanut seeds with HgCl2 and fungicide Abound
Materials
Peanut seeds
Protocol materials
Abound Flowable FungicideSyngenta
Step 4
In a Clean Bench in room 332, put
In a sterile flask, add 150 mL 0.1% Hg2Cl sterilized 0.1% Hg2Cl solution and shake occasionally for 00:07:00 .
7m
Remove the Hg2Cl by pouring it to a specific biohazard collection container.
Rinse the seeds 3 times with 150 mL ddH2O for 00:05:00 each with constant shaking.Collect the rinse water to water waste bottle.
5m
Prepare Abound Flowable FungicideSyngenta by adding 0.6 mL fungicide in 100 mL ddH2O , add the diluted fungicide to the flask. Soak the seeds in the fungicide for 00:03:00 with frequent shaking.
3m
Remove the fungicide by pouring it to a specific biohazard collection container.
Rinse the seeds 3 times with 150 mL ddH2O for 00:05:00 each with constant shaking.Collect the rinse water to water waste bottle.
5m
Add ddH2O to the flask until the seeds are fully merged. Put it in dark growth chamber 26 °C for 48:00:00 .
2d
Pour out the ddH2O, and put the seeds in a germination box