A | B | |
Nuclease Free Water | 17 uL | |
Buffer 10X PCR | 5 uL | |
50 mM MgCl2 | 1.5 uL | |
10 mM dNTP’s | 1 uL | |
10 uM Forward primer | 2.5 uL | |
10 uM Reverse primer | 2.5 uL | |
DNA (25 mg/uL) | 20 uL | |
Taq Platinum Polymerase | 0.5 uL | |
Final Volume | 50 uL |
A | B | C | D | |
1 Cycle | Initial activation | 2 minutes | 94ºC | |
35 Cycles | Denaturation | 30 seconds | 94ºC | |
35 Cycles | Alignment | 30 seconds | 50ºC | |
35 Cycles | Extension | 2 minutes | 72ºC | |
1 Cycle | Final Extension | 5 minutes | 72ºC | |
Maintenance | Maintenance | - | 4ºC |