Apr 29, 2022
Version 4
Patch-Seq Internal Solution with Biocytin V.4
- Allen Institute for Brain Science1
- 1Allen Institute
Protocol Citation: Allen Institute for Brain Science 2022. Patch-Seq Internal Solution with Biocytin. protocols.io https://dx.doi.org/10.17504/protocols.io.261geo4qol47/v4Version created by Allen Institute
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: April 29, 2022
Last Modified: April 29, 2022
Protocol Integer ID: 61706
Keywords: patch-seq, biocytin, RP0235
Abstract
This SOP provides instruction to prepare Internal Solution which is used for patch clamp electrophysiology, modified for cleanliness and mRNA capture and sustainability.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
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