Nov 23, 2023
Passive avoidance (step-down test)
- 1Vall d'Hebron Research Institute
Protocol Citation: Marina Lorente Picón, Núria Peñuelas, Ariadna Laguna, Miquel Vila 2023. Passive avoidance (step-down test). protocols.io https://dx.doi.org/10.17504/protocols.io.5jyl8pkb9g2w/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 21, 2023
Last Modified: November 23, 2023
Protocol Integer ID: 91252
Funders Acknowledgement:
ASAP
Grant ID: ASAP-020505
Abstract
Passive avoidance (step-down test) for mice
Prepare the platform: Passive Avoidance - Step Down for Mice (vibrating platform) (#40570, Ugo Basile) consisting of a platform located in a controllable electrified net.
Train the animals 24h before the test. Put the animal in the plexiglass platform located in the middle of the electrified net with 0.3 mA. If the mouse steps down the platform, it will receive an electric shock. Stop immediately the electrified net and remove the animal from the cage.
Clean the platform each time between animals.
After 24 hours, place again the same mice in the same platform. Do not electrify the net. Measure the time that the animal spends on the platform before stepping down.
Calculate the time on the platform by subtracting the latency time from the first day to that of the second day to normalize for the inter-individual variability in curiosity/activity of each mouse.