Aug 11, 2023

Public workspaceParse Evercode Fixation Protocol v2.0.1 for cells or nuclei -- University of Minnesota TMCs

DOI
dx.doi.org/10.17504/protocols.io.x54v9d6p1g3e/v1
  • Parse Biosciences1,
  • Laura J Niedernhofer2,
  • David A Bernlohr2
  • 1Parse Biosciences, support@parsebiosciences.com;
  • 2University of Minnesota, Minneapolis, MN USA
Mickayla DuFresne-To
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DOI: dx.doi.org/10.17504/protocols.io.x54v9d6p1g3e/v1
Protocol Citation: Parse Biosciences, Laura J Niedernhofer, David A Bernlohr 2023. Parse Evercode Fixation Protocol v2.0.1 for cells or nuclei -- University of Minnesota TMCs. protocols.io https://dx.doi.org/10.17504/protocols.io.x54v9d6p1g3e/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 03, 2023
Last Modified: August 11, 2023
Protocol Integer ID: 81370
Keywords: Parse, Evercode, scRNAseq, snRNAseq, Fixed, sc/snRNAseq, UMN, UMinnesota, University of Minnesota
Funders Acknowledgement:
NIH
Grant ID: 1U54AG079754-01
NIH
Grant ID: 1U54AG07041-01
Abstract
https://www.parsebiosciences.com/products/evercode-whole-transcriptome

Fixation for Parse Evercode sc/snRNAseq
Includes details for both cells and nuclei fixation -- However, each require a different product kit from Parse to complete.
Please check for the most recent version of the protocol before going forward.
Download Parse Evercode Fixation user manual (v2.pdfParse Evercode Fixation user manual (v2.pdf