Preparation (all one day in advance):
Note: 1 set refers to 1x the number of samples + extraction control
- Clean whole extraction hood with bleach, EtOH, Eliminase (this order)
- Clean microtube and falcon racks in the same way and put them in the hood
- Clean pipettes, tip boxes (1x small, 1x medium, 3x large), and hood waste the same way
- Label tubes and columns in the hood and close them after labelling (no detailed labels necessary unless specified)
- 1 set Zymo-Spin ICXM columns + collection tubes (labelled “DNA”)
- 1 set Zymo-Spin IC columns + collection tubes (labelled “RNA”)
- 9 more sets of collection tubes (4 sets labelled “DNA”, 6 sets labelled “RNA”)
- 2 sets 1.5 mL tubes (1 set labelled “DNA”, 1 set labelled “RNA”)
- 2 sets Zymo-Spin III u-HCR Filter columns + collection tubes (1 set labelled “DNA”, 1 set labelled “RNA”)
- 2 sets 1.5 mL tubes with detailed labels (1 set labelled “DNA”, 1 set labelled “RNA”)
- 2 sets Qubit tubes (1 set labelled “DNA”, 1 set labelled “RNA”) + 4 additional Qubit tubes for standards (2 RNA + 2 DNA)
- 2 additional 1.5 mL tubes for DNase-mix preparation + 2 additional 5 mL tubes for Qubit solution preparation (RNA + DNA)
- Put 100% EtOH and 5 mL tubes in falcon rack
- UV-sterilize everything overnight