Protocol Citation: molmer, Martin Lotz 2022. Overview of scRNA-seq of Human Knee Meniscus. protocols.io https://dx.doi.org/10.17504/protocols.io.dm6gpb7p5lzp/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 10, 2022
Last Modified: August 23, 2022
Protocol Integer ID: 68485
Abstract
The overview provides sub-protocols for each step required to complete scRNA-seq from human knee meniscus.
Tissue Acquisition
Single-Cell Isolation
cDNA Library Preparation
Data Processing and Quality Control