Mar 01, 2022
Overall protocol for top-down LC-MS/MS of human spleen tissue
- 1Northwestern University;
- 2Purdue University
Protocol Citation: Jeannie Camarillo, Bryon Drown, Neil Kelleher 2022. Overall protocol for top-down LC-MS/MS of human spleen tissue. protocols.io https://dx.doi.org/10.17504/protocols.io.b5tgq6jw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: March 01, 2022
Last Modified: March 01, 2022
Protocol Integer ID: 58952
Abstract
Overview description of the process for acquiring top-down LC-MS/MS data on human spleen tissue
Tissue Collection
Tissue Collection
Spleen tissue was provided by University of Florida TMC. Tissue was collected and prepared according to the following:
Protocol
NAME
Case Processing SOP (Spleen)
CREATED BY
Marda Jorgensen
Sample Preparation
Sample Preparation
Perform LC based Proteomics on adjacent tissue sections:
Protocol
NAME
Tissue Homogenization and GELFrEE for Top-Down Proteomics
CREATED BY
Kelleher KRG KRG Research Group
Data Acquisition
Data Acquisition
Protocol
NAME
Untargeted Top-down Proteomics by LC-MS/MS on LumosCREATED BY
Kelleher KRG KRG Research Group
Data Analysis
Data Analysis
Protocol
NAME
Top-down proteomics data analysis on the NRTDP TDPortal
CREATED BY
Kelleher KRG KRG Research Group