1. Qubit DNA Quantification Assay kit (High Sensitivity) or Quant-iT PicoGreen dsDNA Assay Kit with Lambda DNA standard (Invitrogen/Thermo Fisher Scientific)
2. Ovation RRBS Methyl-Seq System (for 32 or 96 samples)
3. Unmethylated DNA control (any supplier)
5. Agencourt Magnetic Beads
6. 20X EvaGreen (any supplier) - if doing qPCR
1. A set of standard single-channel pipettes with low retention nuclease-free tips
2. ESSENTIAL Multichannel pipettes: manual 0.5-10 µl, 2-20 µl and 20-200 µl 8-channel pipettes and an electronic dispensing 100-1200 µl 8-channel pipette with a buffer reservoir
3. ESSENTIAL Electronic multi-dispenser pipette, such as Eppendorf Multipette E3 with associated combitips of different sizes
4. Pipet-aid/pipettor with stripettes (for the ethanol wash prep)
5. Clear 0.2 ml PCR strip tubes or clear 96-well PCR plates
6. Adhesive seals for PCR plates and seal applicators
7. Plastic tubes of different sizes – 0.5 ml to 5 ml tubes, and 15- or 50-ml conical centrifuge tubes (for ethanol)
8. Ice bucket with ice or cooling racks and cooling plate stands
1. Qubit fluorometer or a fluorescent microplate reader
2. Magnetic separation rack or plate
4. Benchtop microcentrifuges: for 0.2 ml tubes and plates
5. Bioanalyzer or TapeStation or other fragment analyzer, together with associated reagents and consumables (always use the ‘High Sensitivity’ DNA assays)