a. Add 3mL 10% neutral buffered formalin (NBF) into a 5mL cryovial containing normal donor lymph nodes.
b. Add 10 mL 10% NBF into a labeled 20 mL glass scintillation vial, and place four 1.25 cm3 pieces of tissue from normal donor lung parenchyma into the vial.
c. Top off the vial with NBF if needed and protect it from light. Samples must be submerged for at least 24 hours and no longer than 72 hours to ensure complete saturation and to prevent over-fixation.
d. Remove tissue from NBF and use a scalpel to cut the tissue into a <2mm thick piece that is small enough to fit into the tissue cassette.
e. Enclose tissue into the cassette and then submerge the cassette in 70% ethanol.
f. Embedding and histology are performed using standard methods.
a. Cut the tissue into 1 x 1 cm pieces for freezer preservation in OCT at -80°C.
b. Transfer the piece of tissue into 4% paraformaldehyde at 4°C for 24 h.
c. Transfer to 30% sucrose solution at 4°C for 24 h, then embed in a mold with OCT.
d. Store in a -80°C freezer.
3. For Lipidomics and Proteomics analyses:
a. Cut the tissue into 1 x 1 cm pieces for freezer preservation in 75% of Hydroxypropyl
methylcellulose (HPMC) and 2.5% of polyvinylpyrrolidone (PVP) at -80°C.
b. Embed in a mold with HPMC-PVP.
c. Store in a -80°C freezer.