Oct 03, 2023

Public workspaceOSU TriState SenNet Normal Donor Lung Acceptance Criteria

DOI
dx.doi.org/10.17504/protocols.io.ewov1qzjkgr2/v1
  • 1Comprehensive Transplant Center, Wexner Medical Center, Ohio State University, Columbus, Ohio, USA.;
  • 2Division of Pulmonary, Critical Care and Sleep Medicine, College of Medicine, Ohio State University, Columbus, Ohio, USA.
Lorena Rosas
Icon indicating open access to content
QR code linking to this content
DOI: dx.doi.org/10.17504/protocols.io.ewov1qzjkgr2/v1
Protocol Citation: Sean D. Stacey, Brenda F. Reader, Lorena Rosas, Victor Peters, Ana L. Mora, mauricio.rojas 2023. OSU TriState SenNet Normal Donor Lung Acceptance Criteria. protocols.io https://dx.doi.org/10.17504/protocols.io.ewov1qzjkgr2/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 03, 2023
Last Modified: October 03, 2023
Protocol Integer ID: 88721
Keywords: biospecimen, Human lung tissue, normal donor, TriState SenNET
Funders Acknowledgement:
TriState SenNET (Lung and Heart) Tissue Map and Atlas consortium - NIH
Grant ID: U54AG075931
Lifeline of Ohio and the generous donation from donors and their families
Abstract
This protocol describes the criteria required for inclusion of human lung specimen from healthy “normal” organ donors in the Comprehensive Transplant Center Human Tissue Biorepository and TriState SenNet Biospecimen Core at The Ohio State University as part of the Cellular Senescence Network Program (SenNet). This protocol is based on the CTC Human Tissue Biorepository's standard operating procedure established for tissue collection for explant and donor samples. We follow routine laboratory specimen processing guidelines to ensure the highest biospecimen quality.
Inclusion Criteria
Inclusion Criteria
Donor is consented for research by Lifeline of Ohio or registered organ donor, 18-80 years of age, negative for HCV, HBV, HIV, and no history of latent tuberculosis or active COVID-19 infection. Depending on the investigators’ request we remove specimens from upper, lower, or both lobes of a single lung. When processing “normal” donor lung tissue, we choose samples from the healthiest section of the lung. We refer to the CT scan or chest x-ray to locate these regions.
For “normal” donor lungs, we look for the following characteristics:

1. Tissue is spongy.
2. Tissue is pink in color and color is uniform.
3. Absence of abscesses, nodules, or emphysematous presentations.
Exclusion Criteria
Exclusion Criteria
Donor is 17 years of age or younger, HBV, HCV, or HIV positive, has a latent tuberculosis infection or active COVID-19 infection.