1. Poly-D-lysine (PDL): Sigma #P0899 or ThermoFisher #A3890401
2. Coverslips: can be purchased from any supplier. A few options are: EMS #72230-01, NeuVitro #GG-12-15H
4. Coverslip rack: EMS #70366-16
5. SSC: ThermoFisher #15557044
6. Forceps: any #3 or #5 forcep appropriate for coverslip handling. A few suggestions: FST #11231-30, FST #11252-30
7. Hybridization buffer: can be ordered directly from Molecular Instruments website for the desired volume. Make sure to select the appropriate hybridization buffer for cell culture work.
8. Wash buffer: can be ordered directly from Molecular Instruments website for the desired volume. Make sure to select the appropriate hybridization buffer for cell culture work.
9. Amplification buffer; can be ordered directly from Molecular Instruments website for the desired volume.
10. origamiFISH probes: sequences can be found in Supplementary Table 1 of the origamiFISH paper. The oligos can be ordered directly from IDT as 25nmol or 100nmol format STD - we typically order as an oligo plate. We typically make 100µM stock solutions of each oligo for long-term storage, and then aliquots of probe pool working stocks (i.e. containing all probes) at 1µM. This makes it such that you can add 1µL of working stock probe pool per 100µL hybridization buffer for the probe solution.
11. HCR hairpins: can be ordered directly from Molecular Instruments website for the desired volume and color combinations. The origamiFISH probe sequences provided above correspond to the B2 and B3 HCR initiators. Therefore, if you would like to perform origamFISH in Alexa 647, purchase the B2-647 or B3-647 hairpins and use with the corresponding probes. Likewise if you would like to perform origamiFISH in Alexa 488, purchase the B2-488 or B3-488 hairpins. If you want to perform dual origamiFISH and staple-initiated origamiFISH, simply use B2 for one, and B3 for the other.
12. Fluoromount-G: Southern Biotech #0100-01