Protocol Citation: yzhu 2024. Organoid Drug Treatment. protocols.io https://dx.doi.org/10.17504/protocols.io.x54v92rb1l3e/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 13, 2024
Last Modified: June 13, 2024
Protocol Integer ID: 101794
Abstract
Protocol for drug experiments of organoid samples
Trypsinize organoids and plate at 30,000 cells/well in 24-well plates
Incubate 5-7 days
Incubate organoids in a complete medium containing 500 nM JQ1 for 24 hours (JQ cells) or 0.1% v/v DMSO (control cells)
Harvest organoids in Cell Recovery Solution (Corning) on ice for 1 hour, wash with PBS, and centrifuge to retrieve cell pellets
Proceed to process cell pellets for RNA extraction