Dec 20, 2024

Public workspaceOrgan Harvest Protocol: Intestine V.2

DOI
dx.doi.org/10.17504/protocols.io.14egn9w7zl5d/v2
  • 1Stanford University
Joanna Bi
Icon indicating open access to content
QR code linking to this content
DOI: dx.doi.org/10.17504/protocols.io.14egn9w7zl5d/v2
Protocol Citation: Yiing Lin 2024. Organ Harvest Protocol: Intestine. protocols.io https://dx.doi.org/10.17504/protocols.io.14egn9w7zl5d/v2Version created by Joanna Bi
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 20, 2024
Last Modified: December 22, 2024
Protocol Integer ID: 116757
Abstract
Organ harvesting protocol for HuBMAP small and large intestine.
Donor Demographics and Lab Values
Donor Demographics and Lab Values
Download DonorDemographics.pdfDonorDemographics.pdf155KB

Organ Recovery
Organ Recovery
For small intestine:

Cross-clamp and flush with UW/HTK.
Recover the small intestine.
For large intestine:
If possible, do not ligate the inferior mesentric artery prior to cross-clamp and flush. Cross-clamp and flush with UW/HTK.
Recover the large intestine.
Submerge in chilled UW/HTK - do not wrap in surgical towel. Triple bag the tissue.

Label the specimen bag with project code, tissue, size and donor number
Package on wet ice and maintain at 2-8 °C until shipment. Contact NDRI immediately following procurement.