| REAGENT or RESOURCE | SOURCE | IDNETIFIER |
| Antibodies |
| Mouse anti-HA | Millipore | Cat# 05-904 RRID: AB_417380 |
| Chemicals, peptides, and kits |
| TRIzolTM Reagent | Thermo | Cat# 15596026 |
| Cycloheximide | Sigma-Aldrich | Cat# 1810 |
| Magnesium chloride | Sigma-Aldrich | Cat# M8266 |
| Potassium chloride | Sigma-Aldrich | Cat# P9333 |
| DNase1 | Invitrogen | Cat# 18068015 |
| Pierce™ Protein A/G Magnetic Beads | Thermo | Cat# 88803 |
| Chloroform | Sigma | Cat# C2432 |
| 20X TE Buffer (pH 7.5) | Promega | Cat# A2651 |
| Ethyl alcohol, Pure | Sigma | Cat# E7023 |
| Glycogen, Molecular Biology Grade | Roche | Cat# 10901393001 |
| RNasin®
Ribonuclease Inhibitor | Promega | Cat# N2115 |
| Pierce™ Protein A/G Magnetic Beads | Thermo Fisher Scientific | Cat# 88803 |
| Halt™ Protease and Phosphatase Inhibitor Cocktail | Thermo Fisher Scientific | Cat# 78444 |
| NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® | NEB | Cat# E6420L |
| NEBNext® Multiplex Oligos for Illumina® | NEB | Cat# E7600S |
| High Sensitivity D5000 Screen tape | Agilent | Cat# 5067-5592 |
| High Sensitivity D1000 Screen tape | Agilent | Cat# 5067-5584 |
| High Sensitivity RNA Screentape | Agilent | Cat# 5067-5579 |
| High Sensitivity D5000 Screen tape Reagent | Agilent | Cat# 5067-5593 |
| High Sensitivity D1000 Screen tape Reagent | Agilent | Cat# 5067-5585 |
| High Sensitivity RNA Screen tape Reagent | Agilent | Cat# 5067-5580 |
| High Sensitivity D5000 Screen tape ladder | Agilent | Cat# 5067-5594 |
| High Sensitivity D1000 Screen tape ladder | Agilent | Cat# 5067-5587 |
| High Sensitivity RNA Screen tape ladder | Agilent | Cat# 5067-5581 |
| Glass homogenizer | WHEATON | Cat# 357542 |
| Disposable scalpel | Bard-Parker | Cat# 371611 |
| Experimental models: Organisms/strains | | |
| Mouse: Tie2-Cre | The Jackson Laboratory | Stock# 008863 |
| Mouse: Ai9 | The Jackson Laboratory | Stock# 007909 |
| Mouse:RiboTag mice (Rpl22HA/HA) | The Jackson Laboratory | Stock# 011029 |